TESA & PESA Treatment

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TESA & PESA – Advanced Sperm Retrieval Techniques at Jananam Fertility Centre

At Jananam Fertility Centre, we offer Testicular Sperm Aspiration (TESA) and Percutaneous Epididymal Sperm Aspiration (PESA) —two highly effective surgical sperm retrieval techniques for men facing azoospermia (absence of sperm in the ejaculate).

For men who cannot produce sperm naturally through ejaculation due to blockages, low sperm production, or previous vasectomy, TESA and PESA provide a direct method of retrieving viable sperm, which can then be used in IVF/ICSI treatments to achieve pregnancy.

By combining expert medical care, cutting-edge technology, and a patient-centered approach, we help couples overcome male infertility and take a step closer to parenthood.

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Understanding TESA & PESA – What’s the Difference??

Both TESA and PESA involve surgical sperm extraction, but the approach differs depending on whether the cause of azoospermia is obstructive or non-obstructive.

TESA (Testicular Sperm Aspiration)

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TESA is a minimally invasive technique where a fine needle is inserted into the testicle to extract sperm-containing tissue. The extracted tissue is then processed in the laboratory to retrieve live sperm.

TESA is ideal for:
  • Non-Obstructive Azoospermia (NOA) – When sperm production is low or absent due to testicular dysfunction.
  • Sperm Retrieval for ICSI – Sperm collected via TESA is usually used for Intracytoplasmic Sperm Injection (ICSI).
  • Testicular Dysfunction Cases – Such as men with Klinefelter’s Syndrome or those with prior testicular trauma.

PESA (Percutaneous Epididymal Sperm Aspiration)

PESA is a procedure where a fine needle is used to extract sperm directly from the epididymis— tube where sperm is stored before ejaculation.

PESA is beneficial for:
  • * Obstructive Azoospermia (OA) – When sperm production is normal, but a blockage in the reproductive tract prevents sperm from being released.
  • *Failed Vasectomy Reversal – For men who had a vasectomy but now wish to conceive.
  • * Congenital Absence of Vas Deferens (CAVD) – A genetic condition where men are born without the tube that transports sperm.
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TESA vs. PESA

TESA vs. PESA – A Detailed Comparison

Feature TESA (Testicular Sperm Aspiration) PESA (Percutaneous Epididymal Sperm Aspiration)
Procedure Type Needle inserted into the testicle to extract tissue and sperm Needle inserted into the epididymis to retrieve sperm
Best for Non-obstructive azoospermia (low or no sperm production) Obstructive azoospermia (normal sperm production but blocked ducts)
Anesthesia Used Local anesthesia (mild sedation if needed) Local anesthesia (mild sedation if needed)
Sperm Quality Sperm may be immature and require ICSI Sperm is more mature and motile
Recovery Time 24–48 hours 24 hours
Risk of Pain/Swelling 2Minimal Minimal
Usage in ART Usually combined with ICSI for fertilization Can be used in IVF or ICSI

Who Needs TESA & PESA?

TESA and PESA are recommended for men facing:

PESA is beneficial for:
  • * Obstructive Azoospermia (OA): Blockage in the reproductive tract due to vasectomy, infections, trauma, or genetic absence of vas deferens.
  • * Non-Obstructive Azoospermia (NOA): Poor or absent sperm production caused by hormonal imbalances, testicular failure, or genetic conditions.
  • * Men with Retrograde Ejaculation: A condition where semen enters the bladder instead of exiting through the penis.
  • Failed Vasectomy Reversal Cases: When a vasectomy reversal surgery is unsuccessful, sperm can still be retrieved directly.
  • Men with Low Sperm Count (Severe Oligospermia): If sperm concentration is too low for natural conception, surgical retrieval can be an option.

Step-by-Step TESA & PESA Process at Jananam Fertility Centre

  • Comprehensive medical history and semen analysis to confirm azoospermia.
  • Ultrasound & hormone testing to assess testicular function.
  • Genetic screening if required for conditions like Klinefelter’s Syndrome or Y-chromosome deletions.

  • Anesthesia: Both TESA & PESA are performed under local anesthesia, with mild sedation if necessary.
  • Procedure Duration: The procedure typically takes 20–30 minutes and is day-care based (no hospital stay required).
  • Sperm Extraction:
  • PESA: A fine needle is inserted into the epididymis, and fluid containing sperm is aspirated.
  • TESA: A fine needle is inserted into the testicle to collect sperm tissue, which is processed to extract viable sperm.

  • The collected sperm is processed in our advanced IVF lab.
  • If necessary, sperm is cryopreserved (frozen) for future IVF/ICSI cycles.

  • Patients can resume normal activities within 24–48 hours.
  • Mild discomfort and swelling subsides within a few days.
  • Detailed post-procedure instructions are provided for optimal healing.

Success Rates of TESA & PESA

The success of sperm retrieval depends on the underlying cause of azoospermia:

TESA Success Rate:
  • * Sperm retrieval is 30–50% in non-obstructive azoospermia (depends on testicular function).
  • * 90% success rate in cases of obstructive azoospermia when combined with ICSI.
  • PESA Success Rate:
  • 80–90% success rate in sperm retrieval for obstructive azoospermia.
  • Higher fertilization rates due to the collection of mature sperm.

When combined with IVF/ICSI, TESA & PESA offer promising pregnancy success rates, helping couples conceive despite severe male infertility challenges.

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Lifestyle Tips to Improve Sperm Health Before TESA/PESA

Even if sperm needs to be retrieved surgically, improving overall sperm health can boost success rates.

  • Eat a nutrient-rich diet with plenty of antioxidants (Vitamin C, Zinc, Omega-3s).
  • Avoid smoking, alcohol, and recreational drugs, which can damage sperm DNA.
  • Exercise moderately, but avoid excessive heat exposure to the testes (e.g., saunas, hot tubs).
  • Consider supplements like CoQ10 and L-carnitine for sperm energy and motility.
  • Manage stress through meditation or counseling, as stress can impact sperm quality.

Why Choose Jananam Fertility Centre for TESA & PESA?

  • Expert-Led Procedures – Conducted by highly trained urologists and andrologists.
  • State-of-the-Art IVF Lab – Advanced sperm processing, cryopreservation, and ICSI integration.
  • Personalized Treatment Plans – Tailored for each patient’s unique fertility condition.
  • High Success Rates – Proven track record in sperm retrieval and IVF/ICSI pregnancies.
  • Comprehensive Care & Emotional Support – A stress-free journey with compassionate specialists.

Take the First Step Towards Parenthood

If you are struggling with male infertility or azoospermia, our TESA & PESA procedures can help retrieve viable sperm and give you the opportunity to conceive through assisted reproductive technology.

Unlock Your Unique Journey to Parenthood

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